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American Society of Microbiology ABMM Sample Questions:

1. A patient with a history of chronic sinusitis develops a severe invasive fungal infection involving the nasal sinuses and extending into the orbit. Tissue biopsy reveals broad, aseptate hyphae with irregular branching.
The patient is diabetic and acidotic. The MOST likely causative organism belongs to the genus:

A) Scedosporium
B) Fusarium
C) Aspergillus
D) Mucor or Rhizopus

2. A research laboratory is studying the pathogenesis of respiratory syncytial virus (RSV) infection in infants.
They find that a specific viral surface glycoprotein is primarily responsible for attachment to host cells. This glycoprotein is:

A) Fusion protein (F)
B) Matrix protein (M)
C) Hemagglutinin (H)
D) Neuraminidase (N)

3. During the investigation of a foodborne illness outbreak linked to raw sprouts, several bacterial isolates are obtained from patient stool samples and the implicated food source. Which of the following molecular methods would be MOST useful for establishing a definitive link between the clinical isolates and the food isolate?

A) Pulsed-field gel electrophoresis (PFGE)
B) Antimicrobial susceptibility testing
C) Gram staining and biochemical profiling
D) Serotyping based on O and H antigens

4. A clinical microbiology laboratory is performing quality control on Gram stain reagents. A control slide containing Escherichia coli and Staphylococcus aureus shows both organisms staining purple after the complete staining procedure. The MOST likely cause of this error is:

A) Omission of the iodine mordant.
B) Insufficient application of crystal violet.
C) Insufficient washing after crystal violet.
D) Over-decolorization with alcohol-acetone.

5. A patient with HIV infection and a very low CD4+ T-cell count develops a disseminated infection with a fungus that is commonly found in pigeon droppings. The MOST likely causative agent is:

A) Cryptococcus gattii
B) Saccharomyces cerevisiae
C) Candida glabrata
D) Cryptococcus neoformans

Solutions: